Advertisement

 

 

Ghrelin therapy improves lung and cardiovascular function in experimental emphysema.

Ghrelin therapy improves lung and cardiovascular function in experimental emphysema.
Author Information (click to view)

Rocha NN, de Oliveira MV, Braga CL, Guimarães G, Maia LA, Padilha GA, Silva JD, Takiya CM, Capelozzi VL, Silva PL, Rocco PRM,


Rocha NN, de Oliveira MV, Braga CL, Guimarães G, Maia LA, Padilha GA, Silva JD, Takiya CM, Capelozzi VL, Silva PL, Rocco PRM, (click to view)

Rocha NN, de Oliveira MV, Braga CL, Guimarães G, Maia LA, Padilha GA, Silva JD, Takiya CM, Capelozzi VL, Silva PL, Rocco PRM,

Advertisement
Share on FacebookTweet about this on TwitterShare on LinkedIn

Respiratory research 2017 11 0318(1) 185 doi 10.1186/s12931-017-0668-9
Abstract
BACKGROUND
Emphysema is a progressive disease characterized by irreversible airspace enlargement followed by a decline in lung function. It also causes extrapulmonary effects, such as loss of body mass and cor pulmonale, which are associated with shorter survival and worse clinical outcomes. Ghrelin, a growth-hormone secretagogue, stimulates muscle anabolism, has anti-inflammatory effects, promotes vasodilation, and improves cardiac performance. Therefore, we hypothesized that ghrelin might reduce lung inflammation and remodelling as well as improve lung mechanics and cardiac function in experimental emphysema.

METHODS
Forty female C57BL/6 mice were randomly assigned into two main groups: control (C) and emphysema (ELA). In the ELA group (n=20), animals received four intratracheal instillations of pancreatic porcine elastase (PPE) at 1-week intervals. C animals (n=20) received saline alone (50 μL) using the same protocol. Two weeks after the last instillation of saline or PPE, C and ELA animals received ghrelin or saline (n=10/group) intraperitoneally (i.p.) daily, during 3 weeks. Dual-energy X-ray absorptiometry (DEXA), echocardiography, lung mechanics, histology, and molecular biology were analysed.

RESULTS
In elastase-induced emphysema, ghrelin treatment decreased alveolar hyperinflation and mean linear intercept, neutrophil infiltration, and collagen fibre content in the alveolar septa and pulmonary vessel wall; increased elastic fibre content; reduced M1-macrophage populations and increased M2 polarization; decreased levels of keratinocyte-derived chemokine (KC, a mouse analogue of interleukin-8), tumour necrosis factor-α, and transforming growth factor-β, but increased interleukin-10 in lung tissue; augmented static lung elastance; reduced arterial pulmonary hypertension and right ventricular hypertrophy on echocardiography; and increased lean mass.

CONCLUSION
In the elastase-induced emphysema model used herein, ghrelin not only reduced lung damage but also improved cardiac function and increased lean mass. These findings should prompt further studies to evaluate ghrelin as a potential therapy for emphysema.

Submit a Comment

Your email address will not be published. Required fields are marked *

fifteen − four =

[ HIDE/SHOW ]