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High-efficiency production of human serum albumin in the posterior silk glands of transgenic silkworms, Bombyx mori L.

High-efficiency production of human serum albumin in the posterior silk glands of transgenic silkworms, Bombyx mori L.
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Qian Q, You Z, Ye L, Che J, Wang Y, Wang S, Zhong B,


Qian Q, You Z, Ye L, Che J, Wang Y, Wang S, Zhong B, (click to view)

Qian Q, You Z, Ye L, Che J, Wang Y, Wang S, Zhong B,

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PloS one 2018 01 1913(1) e0191507 doi 10.1371/journal.pone.0191507
Abstract

Human serum albumin (HSA) is an important biological preparation with a variety of biological functions in clinical applications. In this study, the mRNA of a fusion transposase derived from the pESNT-PBase plasmid and a pBHSA plasmid containing the HSA gene under the control of a fibroin light chain (FL) promoter were co-injected into fertilized eggs. Fifty-six transgenic silkworm pedigrees expressing theexogenous recombinant HSA (rHSA) in the posterior silk glands (PSGs) with stable inheritance were successfully obtained. The SDS-PAGE and Western blot results confirmed that the rHSA was secreted into the transgenic silkworm cocoon, and the rHSA could be easily extracted with phosphate-buffered saline (PBS). In our research, the isolated highest amount rHSA constituted up to 29.1% of the total soluble protein of the cocoon shell, indicating that the transgenic silkworm produced an average of 17.4 μg/mg of rHSA in the cocoon shell. The production of soluble rHSA in the PSGs by means of generating transgenic silkworms is a novel approach, whereby a large amount of virus-free and functional HSA can be produced through the simple rearing of silkworms.

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