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Inducible miR-145 expression by HIF-1α protects cardiomyocytes against apoptosis via regulating SGK1 in simulated myocardial infarction hypoxic microenvironment.

Inducible miR-145 expression by HIF-1α protects cardiomyocytes against apoptosis via regulating SGK1 in simulated myocardial infarction hypoxic microenvironment.
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Sun N, Meng F, Xue N, Pang G, Wang Q, Ma H,


Sun N, Meng F, Xue N, Pang G, Wang Q, Ma H, (click to view)

Sun N, Meng F, Xue N, Pang G, Wang Q, Ma H,

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Cardiology journal 2017 10 05() doi 10.5603/CJ.a2017.0105
Abstract
BACKGROUND
Myocardial infarction (MI) is partly due to myocardial cell damage caused by hypoxia. MicroRNAs (miRNAs) have been proved to be closely related to the development and progression of many cardiovascular diseases. This study investigated the role of miR-145 in cardiomyocytes under hypoxic condition.

METHODS
The quantitative real-time polymerase chain reaction (qPCR) was performed to test miR-145 expression in H9c2 cells with HIF-1α abnormal expression under hypoxic condition. The 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyltetrazolium bromide (MTT), Transwell assay and flow cytometry were used to investigate the effects of miR-145 on cell viability, migration and apoptosis under normoxic or hypoxic condition, respectively. Meanwhile, reactive oxygen species (ROS) content in hypoxic H9c2 cells was analyzed. Western blotting was used to explore the potential mechanism of miR-145 protective effects on cardiomyocytes. Expression levels of miR-145 and SGK1 in rat MI model were also assessed.

RESULTS
Results showed that miR-145 was upregulated in H9c2 and HIL-1 cells under hypoxia condition, which was promoted by HIF-1α. MiR-145 overexpression enhanced cell viability and migration under normoxic condition. Under hypoxic condition, miR-145 overexpression promoted cell viability, inhibited apoptosis and ROS activity. Western blotting results proved that miR-145 overexpression inhibited the activation of apoptotic related factors, and promoted activation of PI3K/AKT signaling pathway via SGK1 upregulation. Expression levels of MiR-145 and SGK1 were both upregulated in rat MI models.

CONCLUSIONS
HIF-1α could induce miR-145 upregulation in hypoxic H9c2 and HL-1 cells. MiR-145 protected H9c2 cells against hypoxic damage. SGK1 upregulation and activated PI3K/AKT may have participated in the protective effects of miR-145 on cardiomyocytes.

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