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Inflammatory-linked changes in CpG island methylation of three opioid peptide genes in a rat model for pain.

Inflammatory-linked changes in CpG island methylation of three opioid peptide genes in a rat model for pain.
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Jacobi CLJ, Stein C,


Jacobi CLJ, Stein C, (click to view)

Jacobi CLJ, Stein C,

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PloS one 2018 01 1913(1) e0191698 doi 10.1371/journal.pone.0191698
Abstract

Expression of the opioid peptide genes proopiomelanocortin (Pomc), proenkephalin (Penk), and prodynorphin (Pdyn), in immune cells plays a key role in endogenous pain control. In a rat model of painful unilateral paw inflammation, we isolated cells from popliteal lymph nodes and evaluated the role of CpG island C5-methylation on the transcriptional activation of those genes. Using methylated DNA immunoprecipitation, we sorted gDNA into methylated (me) and non-me fractions and then determined the CpG island methylation status of each fraction via quantitative Real Time-PCR (qRT-PCR). In silico analysis by MethPrimer software identified one CpG island in Pdyn and three each in Pomc and Penk. No substantial changes in C5-methylation of any gene were observed. In conclusion, the CpG island methylation status does not seem to be a key regulator of opioid gene activation in immune cells during peripheral tissue inflammation.

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