Opioid receptors (ORs) are among the best studied G protein-coupled receptors due to their involvement in neurological disorders and important role in pain treatment. Contrary to the classical monomeric model, indirect evidence suggests that ORs might form dimers, which could be endowed with a distinct pharmacological profile, and, thus, be targeted to develop innovative pharmacological therapies. However, direct evidence for the spontaneous formation of OR dimers in living cells under physiological conditions is missing. Despite a growing interest in the kappa opioid receptor (KOR), KOR-selective fluorescent probes are particularly scarce in literature. Herein, we present the first set of fluorescent KOR-selective probes with antagonistic properties. Two of these were employed in single molecule microscopy (SMM) experiments to investigate KOR homodimerization, localization and trafficking. Our findings indicate that most KORs labelled with the new fluorescent probes are present as apparently freely diffusing monomers on the surface of a simple cell model.
[Variables associated with mortality in a selected sample of patients older than 80 years and with some degree of functional dependence hospitalized for COVID-19 in a Geriatrics Service].
August 3, 2020
Linguistic profiles, brain metabolic patterns and rates of amyloid-β biomarker positivity in patients with mixed primary progressive aphasia.
October 5, 2020
- ACC 2020The American College of Cardiology decided to cancel ACC.20/WCC due to COVID-19, which was scheduled to take place March 28-30 in Chicago. However, ACC.20/WCC Virtual Meeting continues to release cutting edge science and practice changing updates for cardiovascular professionals on demand and free through June 2020.
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