MERS-CoV diagnosis: An update.

Author Information (click to view)

Al Johani S, Hajeer AH,

Al Johani S, Hajeer AH, (click to view)

Al Johani S, Hajeer AH,

Share on FacebookTweet about this on TwitterShare on LinkedIn

Journal of infection and public health 2016 4 19() pii 10.1016/j.jiph.2016.04.005


Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient’s testings.

Submit a Comment

Your email address will not be published. Required fields are marked *

9 + 4 =