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MiR-21 ameliorates aged-associated skin wound healing defects in mice.

MiR-21 ameliorates aged-associated skin wound healing defects in mice.
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Long S, Zhao N, Ge L, Wang G, Ran X, Wang J, Su Y, Wang T,


Long S, Zhao N, Ge L, Wang G, Ran X, Wang J, Su Y, Wang T, (click to view)

Long S, Zhao N, Ge L, Wang G, Ran X, Wang J, Su Y, Wang T,

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The journal of gene medicine 2018 04 15() e3022 doi 10.1002/jgm.3022
Abstract
BACKGROUND
The cellular and molecular mechanisms for aged-associated delay of cutaneous wound healing are still not well understood. Our previous results show that miR-21 plays key roles during skin wound healing. We presumed that dysregulation of miR-21 may involve in aged-associated defects in wound healing and miR-21 may be one potential therapeutic target to ameliorate wound defects in elderly subjects.

METHODS
Circular full thickness excisional wounds were made on the dorsal skin of young (2-month-old) and aged (12-month-old) female mice. The wound healing rates were quantified and compared between wild-type and miR-21 knock-in mice. Both histologic and morphometric analyses of the wounds were evaluated. And the expression patterns of miR-21 during wound healing in both young and aged mice were assessed by ISH. The effects of topical miR-21 overexpression on wound healing of aged mice were estimated by both wound closure quantification and histological analyses.

RESULTS
Aged miR-21 knock-in female mice showed significantly improved wound healing comparing with the wild-type counterparts featured with mature granulation tissue, smaller wound width and thinner epidermis. The expression patterns of miR-21 showed miR-21 levels were insufficient in repairing granulation tissue in aged mice. Intradermal injecting miR-21 plasmid around wounds could upregulate miR-21 levels during wound healing and ameliorate aged-associated skin wound defects.

CONCLUSIONS
Our results reveal that upregulating miR-21 levels could improve wound repair in aged mice, which suggests that a therapeutic strategy targeting miR-21 expression in aged-associated wound healing may be feasible.

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