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miR-217 inhibits proliferation, migration, and invasion via targeting AKT3 in thyroid cancer.

miR-217 inhibits proliferation, migration, and invasion via targeting AKT3 in thyroid cancer.
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Lin Y, Cheng K, Wang T, Xie Q, Chen M, Chen Q, Wen Q,


Lin Y, Cheng K, Wang T, Xie Q, Chen M, Chen Q, Wen Q, (click to view)

Lin Y, Cheng K, Wang T, Xie Q, Chen M, Chen Q, Wen Q,

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Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 10 0695() 1718-1724 pii 10.1016/j.biopha.2017.09.074

Abstract
PURPOSE
The aims of this study were to test the influence of miR-217 on the proliferation, invasion, migration of thyroid cancer and the relevant mechanism.

METHOD
miR-217 expression levels in thyroid cancer tissues and cell lines were detected by quantitative real-time PCR (qRT-PCR).Cell Counting Kit-8, flow cytometer, wound healing, transwell invasion assays were applied to evaluate the effect of miR-217 on proliferation, apoptosis, migration and invasion of thyroid cells. The luciferase reporter assay, qRT-PCR, and western blot were used to identify target of miR-217. Relative relationship of expression level between miR-217 and AKT3 was analyzed in thyroid cancer tissues. Xenograft transplantation was performed to test effect of miR-217 in vivo.

RESULTS
We found that the expression of miR-217 was significantly decreased in thyroid cancer tissues cell lines. Significantly, decreased miR-217 expression were associated with the clinical stage and lymph node metastasis. Function studies revealed that miR-217 overexpression in thyroid cancer cells inhibited proliferation, migration, and invasion in vitro, as well as suppressed tumor growth in vivo. Subsequently, AKT3 was identified as a target of miR-217 in thyroid cancer. AKT3 expression was upregulated in thyroid cancer tissues, was inversely correlated with miR-217expression. Besides, overexpression of AKT3 efficiently abrogates suppressive effect on proliferation, migration and invasion in thyroid cancer cells caused by overexpression of miR-217.

CONCLUSION
These data demonstrated a tumor suppressor role for miR-217 in thyroid cancer development and progression by targeting AKT3, suggesting miR-217 might be a potential target for thyroid cancer.

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