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Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda.

Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda.
Author Information (click to view)

Akpaka PE, Kissoon S, Wilson C, Jayaratne P, Smith A, Golding GR,


Akpaka PE, Kissoon S, Wilson C, Jayaratne P, Smith A, Golding GR, (click to view)

Akpaka PE, Kissoon S, Wilson C, Jayaratne P, Smith A, Golding GR,

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PloS one 2017 03 0712(3) e0171317 doi 10.1371/journal.pone.0171317
Abstract

Molecular characteristics of vancomycin resistant enterococci isolates from Bermuda Island is currently unknown. This study was conducted to investigate phenotypic and genotypic characteristics of VRE isolates from Bermuda Island using the chromogenic agar, E-tests, polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Eighteen E. faecium isolates were completely analyzed and were all resistant to vancomycin, susceptible to linezolid and quinupristin/dalfopristin, positive for vanA and esp genes. The MLST analysis confirmed most isolates were of the sequence types linked to clonal complex 17 (CC17) that is widely associated with outbreaks in hospitals. Infection control measures, antibiotic stewardship, and surveillance activities will continue to be a priority in hospital on the Island.

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