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MOLECULAR CLONING AND CHARACTERIZATION OF RIBOSOMAL PROTEIN S9 IN ECHINOCOCCUS GRANULOSUS.

MOLECULAR CLONING AND CHARACTERIZATION OF RIBOSOMAL PROTEIN S9 IN ECHINOCOCCUS GRANULOSUS.
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Wen L, Lü G, Lu S, Gao H, Chen B, Ma Y, Yuan Y, Zhang H, Xiao Y, Liu H, Zhao J, Wang J,


Wen L, Lü G, Lu S, Gao H, Chen B, Ma Y, Yuan Y, Zhang H, Xiao Y, Liu H, Zhao J, Wang J, (click to view)

Wen L, Lü G, Lu S, Gao H, Chen B, Ma Y, Yuan Y, Zhang H, Xiao Y, Liu H, Zhao J, Wang J,

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The Journal of parasitology 2017 09 13() doi 10.1645/16-164
Abstract

Ribosomal protein S9 (RPS9) is an essential functional gene that participate in DNA repair and developmental regulations. A sequence homolog of RPS9 has been found to be upregulated in the protoscoleces (PSCs) of Echinococcus granulosus treated with artemisinin (ART). However, Echinococcus granulosus RPS9 (EgRPS9) has not been identified before. In the present study, the 657 bp cDNA encoding EgRPS9 was cloned. Amino acid sequence analysis showed that EgRPS9 was similar to the RSP9 proteins from Schistosoma japonicum (SjRPS9, 86 %) and Schistosoma mansoni (SmRPS9, 79 %). Phylogenetic tree analysis showed that EgRPS9, SmRPS9 and SjRPS9 were clustered together. We detected the EgRPS9 gene and protein expression in PSCs exposed to artesunate (AS) which displayed a dose – dependent reduction in PSCs viability for 24 hr. The results showed that the EgRPS9 ratio of the 10 μM AS – treated (P < 0.01) -and 40 μM AS - treated (P < 0.05) groups were increased from that of the control group. In addition, the level of reactive oxygen species (ROS) in the AS - treated groups increased in a dose - dependent manner compared to the level in the control group. In conclusion, the expression of EgRPS9 could be induced by ROS and might participate in the oxidative damage - based anti - parasite mechanism of AS treatment.

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