To investigate the diagnostic performance of multicolor imaging for detection of retinal nerve fiber layer (RNFL) defect in myopic eyes with glaucoma.
Comparative diagnostic analysis.
A total of 150 myopic eyes of 138 patients ( 24 mm) underwent multicolor imaging by Spectralis® optical coherence tomography (OCT; Heidelberg Engineering GmbH, Heidelberg, Germany). Two independent observers graded the RNFL visibility score and determined the location of RNFL defect on each image. Readings were taken from the superior and inferior areas. The diagnostic performances of multicolor, green-reflectance, and blue-reflectance images for detection of RNFL defect were compared with those of conventional fundus and red-free RNFL photographs.
The interobserver agreement on RNFL visibility score was high: substantial for fundus and RNFL photographs, multicolor and green-reflectance images, and perfect for blue-reflectance images. The interobserver agreement on location of RNFL defect was better when using multicolor imaging than when using conventional photography. The green-reflectance image (2.89 ± 0.31) showed the highest RNFL visibility score, followed by blue-reflectance (2.88 ± 0.32) and multicolor (2.57 ± 0.63) images. The sensitivity of multicolor imaging (94.9%, 93.2%, and 91.5% for green-reflectance, blue-reflectance, and multicolor images, respectively) was significantly higher than that of fundus photography (83.1%) for detection of superotemporal RNFL defect (all P < 0.05). For inferotemporal defect, it was significantly higher than those of fundus and RNFL photographs (all P < 0.05).
Multicolor imaging can visualize myopic eyes’ glaucomatous RNFL defects that are indistinguishable on conventional photography.

Copyright © 2021. Published by Elsevier Inc.