To review current information on the morphology, functioning, and biochemical makeup of allergic skin was the purpose of this study. It discusses innovative noninvasive methods that have the potential to reveal intimate skin allergy processes in vivo. The epidermal barrier is more than simply a static wrap around the organism; it also functions as a bio-sensor for external danger signals and is a dynamic field for immunological, biophysical, and biochemical activities. Traditional biophysical approaches are supplemented by innovative in-vivo techniques such as Raman spectroscopy, which analyses skin micro composition and develops epidermal profiles. In order to examine the micro-morphological changes in the skin of allergic patients, visualisation methods such as reflectance spectroscopy and optical coherence tomography (OCT) are used.

The noninvasive evaluation of skin functions, micromorphology, biochemistry, and immunological pathways will aid in the understanding of skin allergies. They will enable for the detection of subgroups, such as in atopic dermatitis, and the development of particular treatment modalities.