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Optimization and production of curdlan gum using Bacillus cereus pr3 isolated from rhizosphere of leguminous plant.

Optimization and production of curdlan gum using Bacillus cereus pr3 isolated from rhizosphere of leguminous plant.
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Prakash S, Rajeswari K, Divya P, Ferlin M, Rajeshwari CT, Vanavil B,


Prakash S, Rajeswari K, Divya P, Ferlin M, Rajeshwari CT, Vanavil B, (click to view)

Prakash S, Rajeswari K, Divya P, Ferlin M, Rajeshwari CT, Vanavil B,

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Preparative biochemistry & biotechnology 2018 03 21() doi 10.1080/10826068.2018.1451886

Abstract

Curdlan gum is a neutral water-insoluble bacterial exopolysaccharide composed primarily of linear β-(1,3) glycosidic linkages. Recently, there has been increasing interest in the applications of curdlan and its derivatives. Curdlan is found to inhibit tumors and its sulfated derivative possess anti- HIV activity. Curdlan is biodegradable, non-toxic towards human, environment and edible which makes it suitable as drug-delivery vehicles for sustained drug release. The increasing demand for the growing applications of curdlan requires an efficient high yield fermentation production process so as to satisfy the industrial needs. In this perspective, the present work is aimed to screen and isolate an efficient curdlan gum producing bacteria from rhizosphere of ground nut plant using aniline-blue agar. High yielding isolate was selected based on curdlan yield and identified as Bacillus cereus using GC-FAME analysis. B. cereus PR3 curdlan gum was characterized using FT-IR spectroscopy, SEM and XRD. Fermentation time for curdlan production using B. cereus PR3 was optimized. Media constituents like carbon, nitrogen and mineral sources were screened using Plackett- Burman design. Subsequent statistical analysis revealed that Starch, NHNO, KHPO, NaSO, KHSOand CaClwere significant media constituents and these concentrations were optimized for enhancement of curdlan production up to 20.88 g/L.

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