Advertisement

 

 

Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV.

Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV.
Author Information (click to view)

Brown EP, Weiner JA, Lin S, Natarajan H, Normandin E, Barouch DH, Alter G, Sarzotti-Kelsoe M, Ackerman ME,


Brown EP, Weiner JA, Lin S, Natarajan H, Normandin E, Barouch DH, Alter G, Sarzotti-Kelsoe M, Ackerman ME, (click to view)

Brown EP, Weiner JA, Lin S, Natarajan H, Normandin E, Barouch DH, Alter G, Sarzotti-Kelsoe M, Ackerman ME,

Advertisement

Journal of immunological methods 2018 01 31455() 24-33 pii 10.1016/j.jim.2018.01.013

Abstract

The Fc Array is a multiplexed assay that assesses the Fc domain characteristics of antigen-specific antibodies with the potential to evaluate up to 500 antigen specificities simultaneously. Antigen-specific antibodies are captured on antigen-conjugated beads and their functional capacity is probed via an array of Fc-binding proteins including antibody subclassing reagents, Fcγ receptors, complement proteins, and lectins. Here we present the results of the optimization and formal qualification of the Fc Array, performed in compliance with Good Clinical Laboratory Practice (GCLP) guidelines. Assay conditions were optimized for performance and reproducibility, and the final version of the assay was then evaluated for specificity, accuracy, precision, limits of detection and quantitation, linearity, range and robustness.

Submit a Comment

Your email address will not be published. Required fields are marked *

five × 1 =

[ HIDE/SHOW ]