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Osteogenic induction of bone marrow mesenchymal cells on electrospun polycaprolactone/chitosan nanofibrous membrane.

Osteogenic induction of bone marrow mesenchymal cells on electrospun polycaprolactone/chitosan nanofibrous membrane.
Author Information (click to view)

He Y, Wang W, Tang X, Liu X,


He Y, Wang W, Tang X, Liu X, (click to view)

He Y, Wang W, Tang X, Liu X,

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Dental materials journal 2017 02 22() doi 10.4012/dmj.2016-203
Abstract

A novel chitosan/polycaprolactone (CS/PCL) nanofibrous membrane by electrospinning was developed for guided tissue regeneration (GTR) to improve mechanical properties and to promote osteogenic differentiation. Firstly, chitosan and PCL solutions of different weight ratios (0/100, 30/70, 50/50) were mixed and then electrospun. Our data demonstrated that the CS/PCL (30/70) nanofibrous membrane promoted an increased rBMSCs proliferation when compared to the CS/PCL (50/50) membrane and pure PCL (0/100) membrane. The highest ALP activity and extracellular calcium deposit were observed on the CS/PCL (30/70) nanofibrous membrane, followed by the CS/PCL (50/50) and pure PCL nanofibrous membrane. Furthermore, the expression of osteocalcin (OCN) and Runx2 were also significantly higher on the CS/PCL (30/70, 50/50) nanofibrous membrane as compared to the pure PCL nanofibrous membrane. In conclusion, the electrospun CS/PCL nanofibrous membrane was found to be a biocompatible material that could stimulate osteogenic differentiation, suggesting that the novel CS/PCL membrane has an interesting potential as use for GTR.

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