Advertisement

 

 

Palmitic Acid Induces Müller Cell Inflammation that is Potentiated by Co-treatment with Glucose.

Palmitic Acid Induces Müller Cell Inflammation that is Potentiated by Co-treatment with Glucose.
Author Information (click to view)

Capozzi ME, Giblin MJ, Penn JS,


Capozzi ME, Giblin MJ, Penn JS, (click to view)

Capozzi ME, Giblin MJ, Penn JS,

Advertisement

Scientific reports 2018 04 038(1) 5459 doi 10.1038/s41598-018-23601-1
Abstract

Chronic hyperglycemia is thought to be the major stimulator of retinal dysfunction in diabetic retinopathy (DR). Thus, many diabetes-related systemic factors have been overlooked as inducers of DR pathology. Cell culture models of retinal cell types are frequently used to mechanistically study DR, but appropriate stimulators of DR-like factors are difficult to identify. Furthermore, elevated glucose, a gold standard for cell culture treatments, yields little to no response from many primary human retinal cells. Thus, the goal of this project was to demonstrate the effectiveness of the free fatty acid, palmitic acid and compare its use alone and in combination with elevated glucose as a stimulus for human Müller cells, a retinal glial cell type that is activated early in DR pathogenesis and uniquely responsive to fatty acids. Using RNA sequencing, we identified a variety of DR-relevant pathways, including NFκB signaling and inflammation, intracellular lipid signaling, angiogenesis, and MAPK signaling, that were stimulated by palmitic acid, while elevated glucose alone did not significantly alter any diabetes-relevant pathways. Co-treatment of high glucose with palmitic acid potentiated the expression of several DR-relevant angiogenic and inflammatory targets, including PTGS2 (COX-2) and CXCL8 (IL-8).

Submit a Comment

Your email address will not be published. Required fields are marked *

twenty − 6 =

[ HIDE/SHOW ]