Advertisement

 

 

Physalis Mottle Virus-Like Particles as Nanocarriers for Imaging Reagents and Drugs.

Physalis Mottle Virus-Like Particles as Nanocarriers for Imaging Reagents and Drugs.
Author Information (click to view)

Masarapu H, Patel BK, Chariou PL, Hu H, Gulati NM, Carpenter BL, Ghiladi RA, Shukla S, Steinmetz NF,


Masarapu H, Patel BK, Chariou PL, Hu H, Gulati NM, Carpenter BL, Ghiladi RA, Shukla S, Steinmetz NF, (click to view)

Masarapu H, Patel BK, Chariou PL, Hu H, Gulati NM, Carpenter BL, Ghiladi RA, Shukla S, Steinmetz NF,

Advertisement

Biomacromolecules 2017 11 16() doi 10.1021/acs.biomac.7b01196
Abstract

Platform technologies based on plant virus nanoparticles (VNPs) and virus-like particles (VLPs) are attracting the attention of researchers and clinicians because the particles are biocompatible, biodegradable, noninfectious in mammals, and can readily be chemically and genetically engineered to carry imaging agents and drugs. When the Physalis mottle virus (PhMV) coat protein is expressed in Escherichia coli, the resulting VLPs are nearly identical to the viruses formed in vivo. Here, we isolated PhMV-derived VLPs from ClearColi cells and carried out external and internal surface modification with fluorophores using reactive lysine-N-hydroxysuccinimide ester and cysteine-maleimide chemistries, respectively. The uptake of dye-labeled particles was tested in a range of cancer cells and monitored by confocal microscopy and flow cytometry. VLPs labeled internally on cysteine residues were taken up with high efficiency by several cancer cell lines and were colocalized with the endolysosomal marker LAMP-1 within 6 h, whereas VLPs labeled externally on lysine residues were taken up with lower efficiency, probably reflecting differences in surface charge and the propensity to bind to the cell surface. The infusion of dye and drug molecules into the cavity of the VLPs revealed that the photosensitizer (PS), Zn-EpPor, and the drugs crystal violet, mitoxantrone (MTX), and doxorubicin (DOX) associated stably with the carrier via noncovalent interactions. We confirmed the cytotoxicity of the PS-PhMV and DOX-PhMV particles against prostate cancer, ovarian and breast cancer cell lines, respectively. Our results show that PhMV-derived VLPs provide a new platform technology for the delivery of imaging agents and drugs, with preferential uptake into cancer cells. These particles could therefore be developed as multifunctional tools for cancer diagnosis and therapy.

Submit a Comment

Your email address will not be published. Required fields are marked *

8 + 20 =

[ HIDE/SHOW ]