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Poor performance of two rapid immunochromatographic assays for anti-Japanese encephalitis virus immunoglobulin M detection in cerebrospinal fluid and serum from patients with suspected Japanese encephalitis virus infection in Laos.

Poor performance of two rapid immunochromatographic assays for anti-Japanese encephalitis virus immunoglobulin M detection in cerebrospinal fluid and serum from patients with suspected Japanese encephalitis virus infection in Laos.
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Sengvilaipaseuth O, Castonguay-Vanier J, Chanthongthip A, Phonemixay O, Thongpaseuth S, Vongsouvath M, Newton PN, Bharucha T, Dubot-Pérès A,


Sengvilaipaseuth O, Castonguay-Vanier J, Chanthongthip A, Phonemixay O, Thongpaseuth S, Vongsouvath M, Newton PN, Bharucha T, Dubot-Pérès A, (click to view)

Sengvilaipaseuth O, Castonguay-Vanier J, Chanthongthip A, Phonemixay O, Thongpaseuth S, Vongsouvath M, Newton PN, Bharucha T, Dubot-Pérès A,

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Transactions of the Royal Society of Tropical Medicine and Hygiene 2017 12 13() doi 10.1093/trstmh/trx067
Abstract
Background
Japanese encephalitis virus (JEV) is a leading identified cause of encephalitis in Asia, often occurring in rural areas with poor access to laboratory diagnostics. We evaluated two rapid diagnostic tests (RDTs) for anti-JEV immunoglobulin M (IgM) detection.

Methods
Consecutive cerebrospinal fluid and serum from 388 patients (704 samples) with suspected JEV infections admitted to six hospitals in Laos were tested with one of two SD-Bioline anti-JEV IgM RDTs and the World Health Organization standard anti-JEV IgM enzyme-linked immunosorbent assay (ELISA; Panbio Japanese Encephalitis-Dengue IgM Combo ELISA.

Results and Conclusions
The performance of both RDTs showed strikingly low sensitivity in comparison to anti-JEV IgM antibody capture ELISA (2.1-51.4%), suggesting low sensitivity of the RDTs. We highlight the fundamental prerequisite to validate RDTs prior to use to ensure that they meet standards for testing.

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