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Pro-apoptotic effects of microRNA-365 on retinal neurons by targeting IGF-1 in diabetic rats: an in vivo and in vitro study.

Pro-apoptotic effects of microRNA-365 on retinal neurons by targeting IGF-1 in diabetic rats: an in vivo and in vitro study.
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Zheng K, Wang N, Shen Y, Zhang Z, Gu Q, Xu X, Qin Q, Liu Y,


Zheng K, Wang N, Shen Y, Zhang Z, Gu Q, Xu X, Qin Q, Liu Y, (click to view)

Zheng K, Wang N, Shen Y, Zhang Z, Gu Q, Xu X, Qin Q, Liu Y,

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Journal of diabetes investigation 2018 02 10() doi 10.1111/jdi.12815
Abstract
OBJECTIVE
This study aims to explore the effects of microRNA-365 (miR-365) on apoptosis of retinal neurons by targeting IGF-1 in diabetes mellitus (DM) rats.

METHODS
High glucose-induced retinal neurons were assigned into the blank (with no plasmid transfection), negative control (NC, with plasmid transfection), anti-miR-365(transfected miR-365 antagomir), sh-IGF-1 (transfected IGF-1 shRNA plasmid), and anti-miR-365 + sh-IGF-1 (transfected miR-365 antagomir and IGF-1 shRNA plasmid) groups. Proliferation and apoptosis of retinal neurons were detected by EdU assay and Hoechst 33342 staining, respectively. Expressions of miR-365, IGF-1, Bax, and Bcl-2 were determined by RT-qPCR and western blotting. A control group contained 10 healthy rats. TUNEL staining was used to evaluate apoptosis of retinal neurons in rats.

RESULTS
In the anti-miR-365 group, apoptosis rate and Bcl-2 expression were reduced in comparison with the NC and blank groups, while the sh-IGF-1 and anti-miR-365 + sh-IGF-1 groups presented an opposite trend. Compared with the normal group, expressions of miR-365 and Bax were increased, and expressions of IGF-1 and Bcl-2 were decreased, with more apoptotic cells in DM rat models. The sh-IGF-1 group had lower Bax expression, and higher expressions of IGF-1 and Bcl-2 with fewer apoptotic cells. Additionally, Bax expression was up-regulated, expressions of IGF-1 and Bcl-2 was down-regulated, and apoptotic cells were higher in the anti-miR-365 + sh-IGF-1 groups than the anti-miR-365 group.

CONCLUSION
The results of this study suggest that suppressed miR-365 increases the IGF-1 expression, leading to pro-apoptotic effects on retinal neurons in diabetic rats. This article is protected by copyright. All rights reserved.

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