Journal of diabetes investigation 2018 02 10() doi 10.1111/jdi.12815
This study aims to explore the effects of microRNA-365 (miR-365) on apoptosis of retinal neurons by targeting IGF-1 in diabetes mellitus (DM) rats.
High glucose-induced retinal neurons were assigned into the blank (with no plasmid transfection), negative control (NC, with plasmid transfection), anti-miR-365(transfected miR-365 antagomir), sh-IGF-1 (transfected IGF-1 shRNA plasmid), and anti-miR-365 + sh-IGF-1 (transfected miR-365 antagomir and IGF-1 shRNA plasmid) groups. Proliferation and apoptosis of retinal neurons were detected by EdU assay and Hoechst 33342 staining, respectively. Expressions of miR-365, IGF-1, Bax, and Bcl-2 were determined by RT-qPCR and western blotting. A control group contained 10 healthy rats. TUNEL staining was used to evaluate apoptosis of retinal neurons in rats.
In the anti-miR-365 group, apoptosis rate and Bcl-2 expression were reduced in comparison with the NC and blank groups, while the sh-IGF-1 and anti-miR-365 + sh-IGF-1 groups presented an opposite trend. Compared with the normal group, expressions of miR-365 and Bax were increased, and expressions of IGF-1 and Bcl-2 were decreased, with more apoptotic cells in DM rat models. The sh-IGF-1 group had lower Bax expression, and higher expressions of IGF-1 and Bcl-2 with fewer apoptotic cells. Additionally, Bax expression was up-regulated, expressions of IGF-1 and Bcl-2 was down-regulated, and apoptotic cells were higher in the anti-miR-365 + sh-IGF-1 groups than the anti-miR-365 group.
The results of this study suggest that suppressed miR-365 increases the IGF-1 expression, leading to pro-apoptotic effects on retinal neurons in diabetic rats. This article is protected by copyright. All rights reserved.