Advertisement

 

 

Processing-independent proANP measurement for low concentrations in plasma: reference intervals and effect of body mass index and plasma glucose.

Processing-independent proANP measurement for low concentrations in plasma: reference intervals and effect of body mass index and plasma glucose.
Author Information (click to view)

Mark PD, Hunter I, Terzic D, Goetze JP,


Mark PD, Hunter I, Terzic D, Goetze JP, (click to view)

Mark PD, Hunter I, Terzic D, Goetze JP,

Advertisement

Clinical chemistry and laboratory medicine 2017 07 08() doi 10.1515/cclm-2017-0309
Abstract
BACKGROUND
Decreased concentrations of pro-atrial-derived natriuretic peptides (proABP) in plasma have been associated with obesity and suggested as a predictor of type 2 diabetes. However, assays for measuring proANP are generally aimed to quantitate higher concentrations of proANP associated with cardiac disease. Therefore, we aimed to measure plasma proANP concentrations in a non-obese Scandinavian reference material and evaluate potential associations of plasma proANP with body mass index (BMI) and plasma glucose, respectively.

METHODS
We report an optimized processing-independent assay (PIA) for proANP in the lower concentration range. The assay was optimized by raising the amount of radioactive tracer and modifying the mixing ratio of resuspended plasma and buffer. Blood samples from a Scandinavian plasma cohort of 693 healthy subjects were then analyzed and age and gender-specific reference intervals were determined.

RESULTS
Simple linear regression analyses of proANP and both BMI and plasma glucose in fasting subjects displayed insignificant associations. Multiple regression analyses supported these findings. However, a higher median plasma concentration of proANP was noted among women <50 years compared to men, whereas no gender-specific differences were seen in other age groups. CONCLUSIONS
Our results show that in a healthy non-obese population, BMI and plasma glucose in fasting subjects do not affect plasma proANP concentrations. Our method should be considered for future studies on low proANP concentration studies, e.g. in obesity and diabetes.

Submit a Comment

Your email address will not be published. Required fields are marked *

twelve + 4 =

[ HIDE/SHOW ]