Advertisement

 

 

Proteomic analysis reveals the enhancement of human serum Apolipoprotein A-1(APO A-1) in individuals infected with multiple dengue virus serotypes.

Proteomic analysis reveals the enhancement of human serum Apolipoprotein A-1(APO A-1) in individuals infected with multiple dengue virus serotypes.
Author Information (click to view)

Reddy MN, Dungdung R, Pilankatta R,


Reddy MN, Dungdung R, Pilankatta R, (click to view)

Reddy MN, Dungdung R, Pilankatta R,

Advertisement

Tropical medicine & international health : TM & IH 2017 07 16() doi 10.1111/tmi.12931
Abstract
OBJECTIVES
Human serum protein profiling of the individual infected with multiple dengue virus serotypes for identifying the potential biomarkers and to investigate the cause for the severity of dengue virus infection.

METHODS
Dengue virus NS1 positive serum samples were pooled into two groups (S2 and S3) based on the molecular serotyping and number of heterotypic infections. The pooled serum samples were subjected to two dimensional gel electrophoresis (2DGE) to identify the differentially expressed proteins. The peptide masses of up regulated protein were detected by matrix-assisted laser desorption ionization time-of-flight MALDI-TOF mass spectrometry and analyzed by MASCOT search engine. The results were compared with the control group (S1). The commonly upregulated protein was validated by quantitative ELISA and compared with control as well as single serotypic infected samples.

RESULTS
Based on 2DGE, total thirteen proteins were differentially upregulated in S2 and S3 groups as compared to control. Some of the upregulated proteins were involved in mediating the complement activation of immune response. The Apolipoprotein A-1 (APO A-1) was upregulated in S2 and S3 groups. Upon validation, APO A-1 levels were increased in line with the number of heterotypic infection of dengue viruses.

CONCLUSION
Heterotypic infection of dengue viruses upregulate the serum proteins involved in the complement pathway in the early phase of infection. There was a significant increase in the level of APO A-1 in three different serotypic infections of dengue virus as compared to control. Further, the role of APO-A1 can be explored in elucidating the mechanism of dengue pathogenesis. This article is protected by copyright. All rights reserved.

Submit a Comment

Your email address will not be published. Required fields are marked *

1 + 6 =

[ HIDE/SHOW ]