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Rapid identification of carbapenemase-type blaand ESBL-type blausing multiplex PCR.

Rapid identification of carbapenemase-type blaand ESBL-type blausing multiplex PCR.
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Kayama S, Yano R, Yamasaki K, Fukuda C, Nishimura K, Miyamoto H, Ohge H, Sugai M,


Kayama S, Yano R, Yamasaki K, Fukuda C, Nishimura K, Miyamoto H, Ohge H, Sugai M, (click to view)

Kayama S, Yano R, Yamasaki K, Fukuda C, Nishimura K, Miyamoto H, Ohge H, Sugai M,

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Journal of microbiological methods 2018 03 29() pii S0167-7012(18)30104-0
Abstract

Guiana extended-spectrum (GES) β-lactamases are emerging in Japan. The GES family can be classified into 2 groups, one with extended-spectrum β-lactamase (ESBL)-like activity, which hydrolyzes penicillins and cephalosporins, and the other with carbapenemase-like activity with an extended spectrum toward carbapenems. This difference is mediated by variations in a specific amino acid in the GES protein: G170 N or G170S substitutions. We developed an amplification refractory mutation system (ARMS) PCR assay that enabled rapid identification of these variant genes without sequencing.

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