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Refining a major QTL controlling spotted wilt disease resistance in cultivated peanut (Arachis hypogaea L.) and evaluating its contribution to the resistance variations in peanut germplasm.

Refining a major QTL controlling spotted wilt disease resistance in cultivated peanut (Arachis hypogaea L.) and evaluating its contribution to the resistance variations in peanut germplasm.
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Zhao Z, Tseng YC, Peng Z, Lopez Y, Chen CY, Tillman BL, Dang P, Wang J,


Zhao Z, Tseng YC, Peng Z, Lopez Y, Chen CY, Tillman BL, Dang P, Wang J, (click to view)

Zhao Z, Tseng YC, Peng Z, Lopez Y, Chen CY, Tillman BL, Dang P, Wang J,

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BMC genetics 2018 03 2319(1) 17 doi 10.1186/s12863-018-0601-3
Abstract
BACKGROUND
Spotted wilt, caused by tomato spotted wilt virus (TSWV), has been one of major diseases in cultivated peanut grown in the southeastern United States (US) since 1990. Previously a major quantitative trait locus (QTL) controlling spotted wilt disease resistance was mapped to an interval of 2.55 cM genetic distance corresponding to a physical distance of 14.4 Mb on chromosome A01 of peanut by using a segregating Fpopulation. The current study focuses on refining this major QTL region and evaluating its contributions in the US peanut mini-core germplasm.

RESULTS
Two simple sequence repeat (SSR) markers associated with the major QTL were used to genotype Findividuals, and 25 heterozygous individuals were selected and developed into an Fsegregating population. Based on visual evaluation in the field, a total of 194 susceptible Findividuals were selected and planted into Fgeneration for phenotyping. Nine SSR markers were used to genotype the 194 Findividuals, and QTL analysis revealed that a confidence interval of 15.2 Mb region had the QTL with 22.8% phenotypic variation explained (PVE). This QTL interval was further genotyped using the Amplicon-seq method. A total of 81 non-redundant single nucleotide polymorphism (SNP) and eight InDel markers were detected. No recombinant was detected among the Findividuals. Two InDel markers were integrated into the linkage group and helped to refine the confidence interval of this QTL into a 0.8 Mb region. To test the QTL contributes to the resistance variance in US peanut mini-core germplasm, two flanking SSR markers were used to genotype 107 mini-core germplasm accessions. No statistically significant association was observed between the genotype at the QTL region and spotted wilt resistance in the mini-core germplasm, which indicated that the resistance allelic region at this QTL didn’t contribute to the resistance variance in the US peanut mini-core germplasm, thus was a unique resistance source.

CONCLUSION
A major QTL related to spotted wilt disease resistance in peanut was refined to a 0.8 Mb region on A01 chromosome, which didn’t relate to spotted wilt disease resistance in the US peanut mini-core germplasm and might be a unique genetic source.

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