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ROLE OF AUTONOMIC NERVOUS SYSTEM IN CHRONIC CNS-MEDIATED ANTIDIABETIC ACTION OF LEPTIN.

ROLE OF AUTONOMIC NERVOUS SYSTEM IN CHRONIC CNS-MEDIATED ANTIDIABETIC ACTION OF LEPTIN.
Author Information (click to view)

da Silva AA, Hall JE, Moak SP, Browning J, Houghton HJ, Micheloni GC, do Carmo JM,


da Silva AA, Hall JE, Moak SP, Browning J, Houghton HJ, Micheloni GC, do Carmo JM, (click to view)

da Silva AA, Hall JE, Moak SP, Browning J, Houghton HJ, Micheloni GC, do Carmo JM,

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American journal of physiology. Endocrinology and metabolism 2016 12 06() ajpendo.00301.2016 doi 10.1152/ajpendo.00301.2016
Abstract

This study tested whether chronic ganglionic blockade or hepatic vagotomy attenuates the chronic CNS-mediated antidiabetic and cardiovascular effects of leptin. Male Sprague-Dawley rats were instrumented with telemetry probes and arterial and venous catheters for determination of blood pressure (BP), heart rate (HR), blood sampling and intravenous (IV) infusions. An intracerebroventricular (ICV) cannula was placed into the brain lateral ventricle for infusion of leptin or vehicle. After control measurements, streptozotocin (STZ) was injected IV (50 mg/kg) to induce diabetes and 5 days later leptin (n=6) or saline vehicle (n=5) was infused ICV for 12 days via osmotic pumps. Beginning on day 6 of leptin treatment, the ganglionic blocker hexamethonium (15 mg/kg/day, IV) was infused, while leptin infusion was continued, to assess the role of the autonomic nervous system. Induction of diabetes was associated with increases in blood glucose (98±7 to 350±19 mg/dL), food intake (23±3 to 43±3 g/d), decreases in heart rate (-70±11 bpm), polyuria, and increased water consumption, which were all completely normalized by ICV leptin infusion. Although hexamethonium attenuated leptin’s effect on HR, it failed to impair leptin’s ability to restore euglycemia or to prevent the polyuria or increased water intake in STZ-diabetic rats. We also found that after pretreatment with hexamethonium (n=8), ICV leptin infusion, during continued ganglionic blockade, completely normalized blood glucose in diabetic rats. In addition, selective hepatic vagotomy did not attenuate leptin’s ability to restore euglycemia in diabetic rats. These results suggest that leptin’s powerful chronic CNS antidiabetic actions are mediated primarily via non-autonomic mechanisms.

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