To define the seroepidemiology of pertussis in Hangzhou, to assess pertussis protection levels in healthy people, and to improve pertussis preventive strategies. To choose participants for physical examination at multiple Community Health Centers in Hangzhou, a multistage stratified random selection approach was used. Immunoglobulin G antibodies against pertussis were detected in serum samples using the Enzyme-Linked Immunosorbent Assay (ELISA). The findings were compared across 11 age groups. The relationships between the rates of pertussis IgG seropositivity and the geometric mean concentration (GMC) levels of pertussis IgG and the associated variables were studied using univariate and multivariate analysis. There were 3360 individuals with accessible information, including 1745 males and 1615 females. Of them, 59.6 percent had a definite history of diphtheria–tetanus–pertussis vaccine vaccination (DTP). DTP immunization rates tended to drop as people got older. The seropositivity rate for pertussis IgG was 69.9%, and the GMC for pertussis IgG was 48.46 U/ml. Seropositivity and GMC for pertussis IgG were significantly greater in patients with inoculation vaccine history or unknown history when compared to those without vaccine inoculation, and lower in age groups 10, 20–29, and 30–39 y when compared to the other age groups studied. 

There are distinct distribution patterns of seropositivity and GMC for pertussis IgG for different age groups and vaccination groups’ immunization histories. In order to prevent pertussis, adolescents and adults should receive a booster dose of pertussis vaccination.