This study highlights the potential of surface-enhanced Raman scattering (SERS) to differentiate between B-cell lymphoma (BCL), T-cell lymphoma (TCL), lymph node metastasis of melanoma (Met) and control (Ctr) samples based on the specific SERS signal of DNA extracted from lymph node tissue biopsy. Differences in the methylation profiles as well as the specific interaction of malignant and non-malignant DNA with the metal nanostructure are captured in specific variations of the band at 1005 cm, attributed to 5-methylcytosine and the band at 730 cm, attributed to adenine. Thus, using the area ratio of these two SERS marker bands as input for univariate classification, an area under the curve (AUC) of 0.70 was achieved in differentiating between malignant and non-malignant DNA. In addition, DNA from the BCL and TCL groups exhibited differences in the area of the SERS band at 730 cm, yielding an AUC of 0.84 in differentiating between these two lymphadenopathies. Lastly, using multivariate data analysis techniques, an overall accuracy of 94.7% was achieved in the differential diagnosis between the BCL, TCL, Met and Ctr groups. These results pave the way towards the implementation of SERS as a novel tool in the clinical setting for improving the diagnosis of malignant lymphadenopathy.Copyright © 2021 Elsevier B.V. All rights reserved.
About The Expert
Andrei Stefancu
Vlad Moisoiu
Minodora Desmirean
Stefania D Iancu
Adrian B Tigu
Bobe Petrushev
Ancuta Jurj
Ramona G Cozan
Liviuta Budisan
Bogdan Fetica
Andrei Roman
Gina Dobie
Cristina Turcas
Mihnea Zdrenghea
Patric Teodorescu
Sergiu Pasca
Doina Piciu
Delia Dima
Zoltán Bálint
Nicolae Leopold
Ciprian Tomuleasa
References
PubMed