AIDS research and human retroviruses 2017 02 14() doi 10.1089/AID.2016.0314
This letter comments a recent manuscript published by Feldblum et al. in AIDS RES Hum Retrovir 2016; 32:1067-1071 concerning the use of the SMARTube to detect the acute HIV infection before seroconversion. Reading this manuscript the author remembered the experience performed at more than 25 years ago, when in Brazil she standardized an alternative technique named as "in-vitro induced antibody production" (IVIAP) for diagnosing the HIV infection in adults and in children with >3 months of age; using the same concept of the SMARTube; that is, enhance the synthesis of specific antibodies from HIV-primed B cells, using the innovative technology called "Stimmunology". Also, the author comments to have applied the IVIAP assay in the diagnosis of an atypical case of primary HIV-1 infection before seroconversion, which indicates the same use of the SMARTube in the mentioned manuscript.