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Specific Hypersensitive Response-Associated Recognition of New Apoplastic Effectors from Cladosporium fulvum in Wild Tomato.

Specific Hypersensitive Response-Associated Recognition of New Apoplastic Effectors from Cladosporium fulvum in Wild Tomato.
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Mesarich CH, Ӧkmen B, Rovenich H, Griffiths SA, Wang C, Karimi Jashni M, Mihajlovski A, Collemare J, Hunziker L, Deng CH, van der Burgt A, Beenen HG, Templeton MD, Bradshaw RE, de Wit PJGM,


Mesarich CH, Ӧkmen B, Rovenich H, Griffiths SA, Wang C, Karimi Jashni M, Mihajlovski A, Collemare J, Hunziker L, Deng CH, van der Burgt A, Beenen HG, Templeton MD, Bradshaw RE, de Wit PJGM, (click to view)

Mesarich CH, Ӧkmen B, Rovenich H, Griffiths SA, Wang C, Karimi Jashni M, Mihajlovski A, Collemare J, Hunziker L, Deng CH, van der Burgt A, Beenen HG, Templeton MD, Bradshaw RE, de Wit PJGM,

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Molecular plant-microbe interactions : MPMI 2017 11 16() MPMI05170114FI doi 10.1094/MPMI-05-17-0114-FI
Abstract

Tomato leaf mold disease is caused by the biotrophic fungus Cladosporium fulvum. During infection, C. fulvum produces extracellular small secreted protein (SSP) effectors that function to promote colonization of the leaf apoplast. Resistance to the disease is governed by Cf immune receptor genes that encode receptor-like proteins (RLPs). These RLPs recognize specific SSP effectors to initiate a hypersensitive response (HR) that renders the pathogen avirulent. C. fulvum strains capable of overcoming one or more of all cloned Cf genes have now emerged. To combat these strains, new Cf genes are required. An effectoromics approach was employed to identify wild tomato accessions carrying new Cf genes. Proteomics and transcriptome sequencing were first used to identify 70 apoplastic in planta-induced C. fulvum SSPs. Based on sequence homology, 61 of these SSPs were novel or lacked known functional domains. Seven, however, had predicted structural homology to antimicrobial proteins, suggesting a possible role in mediating antagonistic microbe-microbe interactions in planta. Wild tomato accessions were then screened for HR-associated recognition of 41 SSPs, using the Potato virus X-based transient expression system. Nine SSPs were recognized by one or more accessions, suggesting that these plants carry new Cf genes available for incorporation into cultivated tomato.

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