Advertisement

 

 

SREBP1c mediates the effect of acetaldehyde on Cidea expression in Alcoholic fatty liver Mice.

SREBP1c mediates the effect of acetaldehyde on Cidea expression in Alcoholic fatty liver Mice.
Author Information (click to view)

He Q, Diao Y, Zhao T, Hou B, Ngokana LD, Liang H, Nie J, Tan P, Huang H, Li Y, Qi L, Zhao Y, Liu Y, Gao X, Zhou L,


He Q, Diao Y, Zhao T, Hou B, Ngokana LD, Liang H, Nie J, Tan P, Huang H, Li Y, Qi L, Zhao Y, Liu Y, Gao X, Zhou L, (click to view)

He Q, Diao Y, Zhao T, Hou B, Ngokana LD, Liang H, Nie J, Tan P, Huang H, Li Y, Qi L, Zhao Y, Liu Y, Gao X, Zhou L,

Advertisement
Share on FacebookTweet about this on TwitterShare on LinkedIn

Scientific reports 2018 01 198(1) 1200 doi 10.1038/s41598-018-19466-z
Abstract

Cell death inducing DNA fragmentation factor-alpha-like A (Cidea) is a member of cell death-inducing DFF45-like effector (CIDE) protein. The initial function of CIDE is the promotion of cell death and DNA fragmentation in mammalian cells. Cidea was recently reported to play critical roles in the development of hepatic steatosis. The purpose of present study is to determine the effect of chronic alcohol intake on Cidea expression in the livers of mice with alcoholic fatty liver disease. Cidea expression was significantly increased in the liver of alcohol-induced fatty liver mice. While, knockdown of Cidea caused lipid droplets numbers reduction. Next, we detected the activity of ALDH2 reduction and the concentration of serum acetaldehyde accumulation in our alcohol-induced fatty liver mice. Cidea expression was elevated in AML12 cells exposed to 100uM acetaldehyde. Interestingly, Dual-luciferase reporter gene assay showed that 100 uM acetaldehyde led to the activation of Cidea reporter gene plasmid which containing SRE element. What’s more, the knockdown of SREBP1c suppressed acetaldehyde-induced Cidea expression. Overall, our findings suggest that Cidea is highly associated with alcoholic fatty liver disease and Cidea expression is specifically induced by acetaldehyde, and this up-regulation is most likely mediated by SREBP1c.

Submit a Comment

Your email address will not be published. Required fields are marked *

17 − 13 =

[ HIDE/SHOW ]