The proportion of Enterobacterales with resistance to third-generation cephalosporins is steadily rising worldwide. A fecal culture positive for EPE is a major risk factor for EPE bloodstream infection, requiring treatment with broad-spectrum antibiotics, such as carbapenems. The primary objective of the present study was to investigate the prevalence and characteristics of EPE carriage in travelers from southern Sweden. These travelers were sampled for bacterial diagnostics of TD compared to those of EPE carriage 10 years ago. The sample material was plated on URI-Select agar plates with vancomycin (Bio-Rad, Hercules, CA, USA) and on ChromID ESBL chromogenic agar plates (bioMérieux, Marcy-l’Étoile, France) and incubated at 37°C overnight. Antimicrobial susceptibility testing was done according to the EUCAST disk diffusion test method. As a result we found out that around 84 of three hundred and three patients carried a total of 92 ESBL-producing members of the Enterobacterales.
Also note that, EPE strains were subjected to whole-genome sequencing (WGS). The total prevalence of EPE in tested samples was thus 28%, compared to 24% 10 years earlier (P = 0.33). Among 86 strains available for WGS, 47 different sequence types (STs) were identified, and there were only 5 ST131 strains. As a conclusion it is evident that, a comparatively high proportion of the strains were ExPEC/UPEC, many expressing the virulence genes pap and/or fim.
Ref link- https://aac.asm.org/content/64/12/e01585-20
