Advertisement

 

 

The Canine POMC Gene, Obesity in Labrador Retrievers and Susceptibility to Diabetes Mellitus.

The Canine POMC Gene, Obesity in Labrador Retrievers and Susceptibility to Diabetes Mellitus.
Author Information (click to view)

Davison LJ, Holder A, Catchpole B, O'Callaghan CA,


Davison LJ, Holder A, Catchpole B, O'Callaghan CA, (click to view)

Davison LJ, Holder A, Catchpole B, O'Callaghan CA,

Advertisement

Journal of veterinary internal medicine 2017 02 08() doi 10.1111/jvim.14636
Abstract
BACKGROUND
Diabetes mellitus (DM) in dogs is a common endocrinopathy with a complex genetic architecture. Disease susceptibility in several breeds is associated with polymorphisms in immune response genes, but in the Labrador retriever breed, no genetic associations with DM have been identified. A deletion in the pro-opiomelanocortin (POMC) gene in Labrador retrievers is associated with increased appetite and risk of obesity.

HYPOTHESIS/OBJECTIVES
To characterize the POMC deletion in Labrador retrievers, to develop a simple genetic test for this mutation, and to test the hypothesis that the POMC gene deletion is associated with an increased risk of DM in this breed.

ANIMALS
Sixty-one non-diabetic Labrador retrievers aged >6 years and 57 Labrador retrievers with DM.

METHODS
Case-control genotyping study to compare the frequency of the POMC deletion in dogs with and without DM. After polymerase chain reaction (PCR) and sequencing to characterize the mutation, a PCR-based test was developed and validated using 2 different restriction fragment length polymorphism assays.

RESULTS
A 14-base-pair deletion was confirmed and localized to exon 3 of the canine POMC gene. A PCR-based test for the deletion was successfully developed. There was no association between the presence of the POMC deletion mutation and DM in this population of Labrador retriever dogs (P = .31).

CONCLUSIONS AND CLINICAL IMPORTANCE
This study adds to the existing scientific literature indicating that there is little evidence for a direct link between obesity and DM in dogs.

Submit a Comment

Your email address will not be published. Required fields are marked *

20 − 17 =

[ HIDE/SHOW ]