Predominantly, two animal models are used for AR. The differences between these two models are not fully understood. Moreover, the dose-response relationship to allergens remains unclear.
Mice were sensitized by nasal drops to set up a nasal-only delivery AR model. Five different doses of OVA nasal drops were served to explore the dose-response to allergens. Allergic symptoms, serum antibodies, spleen supernatant, NALF cytokines, and infiltrated eosinophils of the nasal mucosa were observed.
The allergic symptoms, serum antibodies, cytokines, and infiltrated eosinophils were significantly higher in the high OVA concentration than those of the control group. Different OVA concentrations are associated with the severity of the allergy. OVA concentration is positively related to the severity of symptoms, IgE antibody level, and Th2 bias within a specific concentration range. Meanwhile, serum antibodies and cytokines were significantly higher in the classical IP injection group than in the nasal drip groups.
The IP injection and nasal-only delivery models are two typical AR models that cause a different immune response. A positive dose-response relationship in the nasal-only delivery model is observed from 25 mg/mL to 0.025 mg/mL.
Reference: https://journals.sagepub.com/doi/full/10.1177/1945892418817221
