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The construction and application of a cell line resistant to novel subgroup avian leukosis virus (ALV-K) infection.

The construction and application of a cell line resistant to novel subgroup avian leukosis virus (ALV-K) infection.
Author Information (click to view)

Mingzhang R, Zijun Z, Lixia Y, Jian C, Min F, Jie Z, Ming L, Weisheng C,


Mingzhang R, Zijun Z, Lixia Y, Jian C, Min F, Jie Z, Ming L, Weisheng C, (click to view)

Mingzhang R, Zijun Z, Lixia Y, Jian C, Min F, Jie Z, Ming L, Weisheng C,

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Archives of virology 2017 10 06() doi 10.1007/s00705-017-3563-2

Abstract

A novel avian leukosis viruses (ALV) subgroup named ALV-K was recently isolated from Chinese indigenous chickens which is different from the subgroups (A to E and J) that have previously been reported to infect chickens. More and more ALV-K strains have recently been isolated from local breeds of Chinese chickens. However, there are no more effective diagnostic methods for ALV-K other than virus isolation followed by envelope gene sequencing and comparison. Viral infection can be blocked through expression of the viral receptor-binding protein. In this study, we have engineered a cell line, DF-1/K, that expresses ALV-K env protein and thereby confers resistance to ALV-K infection. DF-1/K can be used in combination with the ALV-K susceptible cell line DF-1 as a specific diagnostic tool for ALV-K and provides a good tool for further research into the molecular mechanisms of interaction between ALV-K env protein and the host cell receptor.

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