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The role of DNA polymerase chain reaction, culture and histopathology in the diagnosis of cutaneous tuberculosis.

The role of DNA polymerase chain reaction, culture and histopathology in the diagnosis of cutaneous tuberculosis.
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Agarwal P, Singh EN, Agarwal US, Meena R, Purohit S, Kumar S,


Agarwal P, Singh EN, Agarwal US, Meena R, Purohit S, Kumar S, (click to view)

Agarwal P, Singh EN, Agarwal US, Meena R, Purohit S, Kumar S,

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International journal of dermatology 2017 08 09() doi 10.1111/ijd.13708

Abstract
BACKGROUND
Cutaneous tuberculosis (TB) is re-emerging as a significant problem in India due to increased incidence of human immunodeficiency virus (HIV), acquired immune deficiency syndrome (AIDS), other immunosuppressive states/factors like drugs, and emergence of multidrug resistance (MDR-TB). Proper diagnosis is warranted as there is varied clinical presentation and similarity with many other diseases.

OBJECTIVE
To assess the sensitivities of DNA polymerase chain reaction (PCR), culture, and histopathology for the diagnosis of cutaneous TB in clinically suspected skin lesions.

METHODS
Clinically suspected cases of cutaneous TB were enrolled in the study. Two skin punch biopsies were taken from each patient and sent for histopathology examination and DNA PCR with culture, respectively.

RESULTS
Of the total 70 cases enrolled, 49 were detected as cutaneous TB by the above tests. The sensitivities of DNA PCR, culture, and histopathology were 24.5, 16.3, and 91.8%, respectively.

CONCLUSION
DNA PCR and culture should be advised in cases of cutaneous TB with inconclusive histopathology. DNA PCR should be considered in preference over culture if facilities are available as it gives rapid results and has a higher sensitivity.

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