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Transcription factor E2F3a regulatestranscription and enhances sensitivity to chemotherapeutic drugs in acute lymphoblastic leukemia.

Transcription factor E2F3a regulatestranscription and enhances sensitivity to chemotherapeutic drugs in acute lymphoblastic leukemia.
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Liu FF, Wang KL, Deng LP, Liu X, Wu MY, Wang TY, Cui L, Li ZG,


Liu FF, Wang KL, Deng LP, Liu X, Wu MY, Wang TY, Cui L, Li ZG, (click to view)

Liu FF, Wang KL, Deng LP, Liu X, Wu MY, Wang TY, Cui L, Li ZG,

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Cancer cell international 2018 03 2018() 40 doi 10.1186/s12935-018-0531-1

Abstract
Background
Low expression ofand caspase 8 associated protein 2 () are associated with poor prognosis of childhood acute lymphoblastic leukemia (ALL).

Methods
Dual-luciferase reporter assay and wild type as well as four mutated types of reporter plasmids were used to demonstrate the activation of E2F3a ontranscription. The direct binding of E2F3a with the promoter ofwas shown by Chromatin Immunoprecipitation (ChIP). Cell proliferation activity and cell cycle were determined by MTS and flow cytometry in leukemic cells after treating with common chemotherapeutic drugs vincristine and daunorubicin.

Results
In this study, we found that up-regulation of E2F3a in leukemic cells led to increased fraction of cells in S and G2/M phase, accelerated proliferation, and enhanced sensitivity to vincristine and daunorubicin. ChIP and luciferase assay indicated that E2F3a could directly bind to two fragments in the wild type ofpromotor (- 206 to - 69 and - 677 to - 507), and activate its transcription activity which was reduced in mutated promotors. The effect of E2F3a on chemotherapeutic sensitivity of leukemic cells could be reversed by down-regulating.

Conclusions
E2F3a could promote transcription and expression of. The effect of E2F3a on chemotherapeutic sensitivity of ALL cells was implemented by regulatingexpression to a great extent.

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