Advertisement

 

 

Understanding of the functional role(s) of the Activating Transcription Factor 4(ATF4) in HIV regulation and production.

Understanding of the functional role(s) of the Activating Transcription Factor 4(ATF4) in HIV regulation and production.
Author Information (click to view)

Lee SD, Yu KL, Park SH, Jung YM, You JC,


Lee SD, Yu KL, Park SH, Jung YM, You JC, (click to view)

Lee SD, Yu KL, Park SH, Jung YM, You JC,

Advertisement

BMB reports 2018 04 11() pii

Abstract

The activating transcription factor (ATF) 4 belongs to the ATF/CREB (cAMP Response Element Binding bZIP (Basic Leucine Zipper)) transcription factor family, and plays a central role in the UPR (Unfolded Protein Response) process in cells. The induction of ATF4 expression has previously been shown to increase the replication of HIV-1. However, the detailed mechanism underlying this effect and the factors involved in the regulation of ATF4 function are still unknown. Here, we demonstrate first that knocking out ATF4 using siRNA shows a strong negative effect on HIV-1 production, indicating that ATF4 is a functional positive cellular factor in HIV-1 production. To determine the mechanism by which ATF4 regulates the HIV-1 life cycle, we assessed the effect of the overexpression of wild type ATF4 and its various derivatives on HIV-1 LTR-mediated transcriptional activation and the production of HIV-1 particles. This effect was studied through co-transfection experiments with either reporter vectors or proviral DNA. We found that the N-terminal domains of ATF4 are involved in HIV-1 LTR-mediated transcriptional activation, and thus in HIV-1 production.

Submit a Comment

Your email address will not be published. Required fields are marked *

thirteen − four =

[ HIDE/SHOW ]