Advertisement

 

 

Vascular smooth muscle cells direct extracellular dysregulation in aortic stiffening of hypertensive rats.

Vascular smooth muscle cells direct extracellular dysregulation in aortic stiffening of hypertensive rats.
Author Information (click to view)

Hays TT, Ma B, Zhou N, Stoll S, Pearce WJ, Qiu H,


Hays TT, Ma B, Zhou N, Stoll S, Pearce WJ, Qiu H, (click to view)

Hays TT, Ma B, Zhou N, Stoll S, Pearce WJ, Qiu H,

Advertisement

Aging cell 2018 03 30() e12748 doi 10.1111/acel.12748
Abstract

Aortic stiffening is an independent risk factor that underlies cardiovascular morbidity in the elderly. We have previously shown that intrinsic mechanical properties of vascular smooth muscle cells (VSMCs) play a key role in aortic stiffening in both aging and hypertension. Here, we test the hypothesis that VSMCs also contribute to aortic stiffening through their extracellular effects. Aortic stiffening was confirmed in spontaneously hypertensive rats (SHRs) vs. Wistar-Kyoto (WKY) rats in vivo by echocardiography and ex vivo by isometric force measurements in isolated de-endothelized aortic vessel segments. Vascular smooth muscle cells were isolated from thoracic aorta and embedded in a collagen I matrix in an in vitro 3D model to form reconstituted vessels. Reconstituted vessel segments made with SHR VSMCs were significantly stiffer than vessels made with WKY VSMCs. SHR VSMCs in the reconstituted vessels exhibited different morphologies and diminished adaptability to stretch compared to WKY VSMCs, implying dual effects on both static and dynamic stiffness. SHR VSMCs increased the synthesis of collagen and induced collagen fibril disorganization in reconstituted vessels. Mechanistically, compared to WKY VSMCs, SHR VSMCs exhibited an increase in the levels of active integrin β1- and bone morphogenetic protein 1 (BMP1)-mediated proteolytic cleavage of lysyl oxidase (LOX). These VSMC-induced alterations in the SHR were attenuated by an inhibitor of serum response factor (SRF)/myocardin. Therefore, SHR VSMCs exhibit extracellular dysregulation through modulating integrin β1 and BMP1/LOX via SRF/myocardin signaling in aortic stiffening.

Submit a Comment

Your email address will not be published. Required fields are marked *

four × 5 =

[ HIDE/SHOW ]