Tropical medicine and health 2018 02 1246() 4 doi 10.1186/s41182-018-0086-9
Multidrug-resistantis an important cause of morbidity and mortality in developing countries. The aim of this study was to characterize and compare multidrug-resistantserovar Typhimurium isolates from patients and poultry feces.
strains were isolated from poultry and patients using standard bacteriological methods described in previous studies. The strains were serotype according to Kaufmann-White scheme and tested for antibiotic susceptibility to 12 different antimicrobial agents using the disk diffusion method. The whole genome of theTyphimurium isolates was analyzed using Illumina technology and compared with 20 isolates ofTyphimurium for which the ST has been deposited in a global MLST database.The ResFinder Web server was used to find the antibiotic resistance genes from whole genome sequencing (WGS) data. For comparative genomics, publicly available complete and draft genomes of differentTyphimurium laboratory-adapted strains were downloaded from GenBank.
All the testedserotype Typhimurium were multiresistant to five commonly used antibiotics (ampicillin, chloramphenicol, streptomycin, sulfonamide, and trimethoprim). The multilocus sequence type ST313 was detected from all the strains. Our sequences were very similar toTyphimurium ST313 strain D23580 isolated from a patient with invasive non-typhoid(NTS) infection in Malawi, also located in sub-Saharan Africa. The use of ResFinder web server on the whole genome of the strains showed a resistance to aminoglycoside associated with carriage of the following resistances genes:,, and; resistance to β-lactams associated with carriage of agenes; resistance to phenicol associated with carriage ofgene; resistance to sulfonamide associated with carriage ofandgenes; resistance to tetracycline associated with carriage ofgene; and resistance to trimethoprim associated togene for all the isolates
The poultry and human isolates were genetically similar showing a potential food safety risk for consumers. Our finding of multidrug-resistantTyphimurium ST313 in poultry feces calls for further studies to clarify the potential reservoirs of this emerging pathogen.