The incidence of cytomegalovirus (CMV) caused or complex colitis among pediatric patients is unknown, even though it is vital to identify. This research aimed to determine the frequency of CMV colitis verified by routine histology in pediatric patients diagnosed with colitis. A review was conducted on the pathology reports generated at Texas Children’s Hospital (TCH) between January 1, 2011, and November 6, 2019. Around 11 individuals out of 1,801 cases of histologic colitis were determined to have CMV by histology. The patients had a mean age of 15.4, and 72.7% were female. The incidence was 0.6%. Around 9 of these 11 patients, or 81.8%, had weakened immune systems, and 4 of them, or 36.4%, had inflammatory bowel disease (IBD) as an underlying diagnosis; of these, 2 had new-onset ulcerative colitis. None of the patients diagnosed with CMV colitis developed a relapse or severe consequences during the average 3.7-year follow-up period (such as colectomy). There was no histologic evidence of CMV found in any of the 54 consecutive cases of IBD-associated colectomy that were analyzed independently at TCH. Researchers concluded that CMV-associated colitis was proven by standard histology in pediatric patients, and IBD-colon explants were uncommon.