The main objective of this study being conducted was to observe the dynamics of blaKPC-2 Dissemination from Non-CG258 Klebsiella pneumoniae to Other Enterobacterales via IncN Plasmids in an Area of High Endemicity. The most important mechanism for carbapenem resistance is the production of carbapenemases. Complex components of blaKPC dispersal have been reported in Colombia, a nation with a high endemicity of carbapenem obstruction. Genomic qualities of KPC-delivering Enterobacterales recuperated from patients infected/colonized and remade the elements of dispersal of blaKPC-2 utilizing both short and long read sequencing, was identified during this study. Therefore, as a result it was found that spread of blaKPC-2 among Enterobacterales in the participating hospitals was due to intra- and interspecies horizontal gene transfer (HGT) mediated by promiscuous plasmids associated with transposable elements that was originated from a multispecies outbreak of KPC-producing Enterobacterales in a neonatal intensive care unit. The plasmids were detected in isolates recovered in other units within the same hospital and nearby hospitals. The gene “epidemic” was driven by IncN-pST15-type plasmids carrying a novel Tn4401b structure and non-Tn4401 elements (NTEKPC) in Klebsiella spp., Escherichia coli, Enterobacter spp., and Citrobacter spp.

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