For a study, researchers sought to efficiently quantify the viability, achievability, and security of Endoscopic ultrasound (EUS) fine-needle biopsy (FNB) in diagnosing gastrointestinal (GI) subepithelial lesions (SELs). Practical examinations were looked at in PubMed and EMBASE and distributed after January 2015 were incorporated. The general paces of demonstrative yield, technical achievement, and unfriendly occasions were determined as result measures. The Jadad scale and the Newcastle-Ottawa scale were utilized to assess the nature of the preliminaries, channel plots and Egger’s test were utilized to gauge distribution inclination, and awareness and subgroup examinations were performed to investigate the fluctuation of heterogeneity and responsiveness separately. About 16 investigations dissecting 969 patients were incorporated in the range between 2015 and 2020. Studies showed little change in responsiveness, and 13 were viewed as excellent. A specific level of distribution predisposition existed in the demonstrative exactness rate. The general paces of demonstrative yield, specialized achievement, and unfriendly occasions were [85.69% (95% CI: 82.73-88.22, I2=41.8%), 98.83% (95% CI: 96.73-99.97, I2=54.3%), and 1.26% (95% CI: 0.35-2.54, I2=0.0%)]. No affecting clinical elements were distinguished in the subgroup examination. EUS-FNB was a promising innovation with a generally prevalent demonstrative yield, technical achievement, and security, which is an ideal choice for finding SELs.