The goal of this review article is to explain 24-hour urine collection in terms of usefulness, collection procedure, and frequent issues encountered during collection. Although 24-hour urine collections are standard of treatment for high-risk stone formers, many subtleties in test acquisition, such as 50% incorrect urine collections and low patient compliance, restrict its potential value. Patients who have not had surgical treatment for urinary calculi, patients with metabolic stone disease or a family history of stone disease, Caucasian ethnicity, and those with more sedentary professions have demonstrated greater compliance in taking 24-hour urine samples. According to studies, there is a disparity in compliance statistics based on patient age and gender.To best use this tool in directing clinical care for the treatment of nephrolithiasis, physicians must first recognise the challenges associated with 24-hour urine collections, such as patient compliance, variability between collections, and complications with interpretation.