The present study aimed to assess the immunogenicity and persistence of antibodies in adults eight years after vaccination. A total of 309 participants, including 94 participants in the isolated anti-HBs group and 215 in the control group, were recruited. All subjects received three doses of hepatitis B vaccine at 0, 1, and 12 months, followed by testing for serological responses one month after the third vaccination. Subsequently, 154 participants were excluded because their anti-HBs data of 8 y after the first vaccination were missing. The prevalence of isolated anti-HBc was about 11.5%. The positive seroprotection rate was 72%. The GMT value of anti-HBs titer was 24.55 mIU/mL in the isolated anti-HBc group 8 y after three doses of vaccination. Researchers detected no significant difference in the positive seroprotection rate and the GMT values of anti-HBs titers between the isolated anti-HBc and control groups after eight years. In conclusion, remote anti-HBc-positive subjects could achieve satisfactory long-term immune effects after hepatitis B vaccination. The GMT values of anti-HBs titers were lower than those of the control group at one month, but Researchers detected no significant difference after eight years.