For a study, researchers sought to estimate proinflammatory markers in night shift workers and determine their relationship to different metabolic syndrome criteria. The study enlisted the participation of 303 people. Demographic information and parameters related to the development of metabolic syndrome were collected. The highly sensitive C-reactive protein (Hs CRP) was studied as a pro-inflammatory marker. FBS, serum triglyceride (TG), and high-density lipoprotein (HDL) levels were measured. The National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III) guidelines determined metabolic syndrome criteria. Night shift workers had a higher hs CRP than day shift workers. TG and FBS levels were significantly elevated (P<0.001). The waist circumference of 6.5% of night-shift workers was greater than 40 inches. Night shift workers with higher hsCRP had significantly higher waist circumference (P<0.001) and FBS (P<0.05). With 3 criteria met, 3.57% of night-shift workers were diagnosed with metabolic syndrome. Working the night shift was associated with increased pro-inflammatory markers and the development of risk factors for metabolic syndrome. Thus, early risk factor screening and management among night shift health care workers might improve their health and prevent the development of MS.