TNBC has a high incidence among young women [5] and the course of the disease is characterized by a high risk of recurrence in the first three years after initial diagnosis. This study was conducted to analyse Optimized tumour infiltrating lymphocyte assessment for triple negative breast cancer prognostics and aimed to establish the optimal assessment method for immunohistochemically stained TILs in TNBC. Approximately 15% of all breast cancers test negative for these three receptors, hence referred to as triple negative breast cancers (TNBC).

 About one fourth of TNBC patients will develop a recurrence after which the median survival is only 9 to 13 months.Being part of the tumour microenvironment, tumour infiltrating lymphocytes (TILs) have gained particular attention. Because of the laborious analysis methods used in the present study, a subcohort of 100 patients (Figure 1) was selected from the previously established TNBC cohort (see further under statistical analysis). Patients who did not develop a recurrence were censored at the date of last follow up. Of every TNBC tissue block, one section was cut to perform the multiplex staining and one for a conventional H&E staining.

To conclude we can say that, Larger studies are needed to find out if one of the used image analysis algorithms is superior in predicting survival.To optimize comparison between markers, we used mIHC combined with spectral imaging.

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