Surgery using the Mohs micrographic technique is typically well-tolerated and results in minimal scarring. Though many different perioperative practices are used to lower the risk of complications, their implementation varies widely, and there is a dearth of data on their effectiveness. The purpose of this study was to analyze the perioperative practices of dermatologic surgeons with regard to antibiotic prophylaxis (AP), anticoagulation, activity restrictions, and antiseptic selection. A total of 2 surveys were distributed by the American College of Mohs Surgery (ACMS) and the American Society for Mohs Surgery (ASMS) to their membership via email. About 177 surgeons took part; among them, 61% were members of ACMS, 35% ASMS, and 4% of both. Preoperatively, 96% (162/168) of surgeons prescribe systemic AP, and postoperatively, 91% (161/177) do so, with the percentages varying according to the surgeon. Preventive aspirin (30%, 45/149), nonsteroidal anti-inflammatory drugs (25%, 36/145), and supplements known to have an anticoagulant effect (54%, 80/149) are more commonly held than therapeutic antiplatelet and anticoagulant medications (3%-5%, 4-7/149). Restrictions on activities and the types of antiseptics prescribed can vary widely. Dermatologic surgeons’ pre- and post-op procedures may differ from standard practice depending on a number of factors. The results of this study highlight the importance of standardization and updated guidelines for perioperative practices in dermatologic surgery.