Radiation-instigated afferent baroreflex failure (R-ABF) occurs because of the wound in the afferent appendage of the baroreflex from radiation in the neck. Recognising and managing R-ABF is tough. We intended to examine the shape of autonomic dysfunction on normalized autonomic testing in patients with likely R-ABF. We reflectively broke down all autonomic reflex screens performed at Mayo Clinic in Rochester, Minnesota, somewhere in the range of 2000 and 2020 in patients with likely R-ABF. Extra tests evaluated included ambulatory BP observing, plasma norepinephrine, and thermoregulatory sweat test. We could classify 90 patients with plausible R-ABF. Median total composite autonomic severity result was 7 (range is 0–10 and IQR is 6–7). Of them, 94.4% patients (i.e. 85) showed signs of cardiovascular adrenergic impairment, 71 patients had higher blood pressure recovery time after Valsalva maneuver, and 68 patients had orthostatic hypotension. The vagal baroreflex sensitivity (57.9%), irregular heart rate responses to deep breathing (79.5%), and Valsalva ratio (87.2%) showed cardiovagal impairment. Ambulatory BP observations showed hypertension, tachycardia, hypertensive surges, postural hypotension, and absence of nocturnal dipping. Weakened vagal baroreflex function and BP lability were correlated. Postganglionic sympathetic sudomotor function was ordinary by and large. Focal neck anhidrosis (78.9%) was the most regular thermoregulatory sweat test verdict. Normalized autonomic testing in R-ABF shows cardiovascular adrenergic hindrance with orthostatic hypotension, pulse lability, and raised plasma norepinephrine. Cardiovagal disability is normal, while sudomotor deficiencies are restricted to straight radiation impacts.