To see if the TNF-, IL-2, IL-4, and IL-10 genes contribute to variances in vaccine-induced immune responses, a total of 369 people who got the inactivated Japanese encephalitis vaccine (IJEV) were enrolled. Individuals were separated into seropositive (SP) and seronegative (SN) groups based on Japanese encephalitis virus (JEV) neutralising antibodies (NAbs). The TaqMan approach was then used to genotype 17 SNPs in the TNF-, IL-2, IL-4, and IL-10 genes. Although TNF-α, IL-2 and IL-4 genes, as well as IL-10 genes, were not associated with JEV seropositivity in the SP group and in the SN group, differences in a subgroup analysis were found. In the male group, the IL-4 gene of rs2243291 was more JEV Seropositive to C/G and in TNF-α gene the Ct generation of rs3093712 was more JEV Natural genotype than the T TT genotype, and the TT genotype (GMT) was higher than the JEV mean titre (GMT) of the TNF-α gene.

In addition, there was an associated tendency in JEV seropositivity in the female group in the rs1800629 genotype and in the TNF-α gene and the rs1800896 genotype in the IL-10 gene. The current investigation has shown that cytokine polymorphisms may be related with the JEV NAbs seroconversion in the sexes. There should yet be extra samples analysed and additional functional verification.