When compared with healthy persons, for a study, the research of the human intestinal microbiome in patients with inflammatory bowel disease (IBD) consistently revealed that there were changes (an aberrant or unbalanced microbiome, “dysbiosis”). The researchers wanted to determine how alterations in the microbiome in individuals changed over time and what clinical parameters were linked to substantial changes. A total of 42 mucosal biopsies were taken from 20 individuals separated by an average of 2.4 years. High-throughput sequencing technologies for the bacterial 16S rRNA gene were used to examine them. Endoscopic and histological examinations were used to identify the presence of active inflammation. The PICRUSt software was used to perform inferred metagenomics analysis. The Yue and Clayton theta distance across sample pairs revealed that variations in the microbiota over time in individuals were most prominent in the presence of persistent intestinal inflammation (adjusted P=0.00031). Patients who had previously undergone abdominal surgery exhibited lower alpha (within-sample) diversity than those who had not (mean Shannon index 2.083, 2.510, respectively, P=0.017). The inferred bacterial metagenomic profile remained unchanged. The microbiota of people with IBD changed a lot over time. When there was histologically proven inflammation at both time points, these alterations were the most pronounced