For a study, it was determined that a major global problem that typically begins in childhood and affects minorities more frequently than Whites is Obesity. As a result, it is essential to identify parameters that might be used as objectives for obesity indicators. The researchers tried to create a profile of gut and oral microbial clades to predict illness status in African American (AA) and European American (EA) children. In both ethnic groups, salivary amylase, socioeconomic factors (education and family income), and obesity were linked to 16S rDNA sequencing of gut and saliva microbial profiles. There were substantial variations in alpha-, beta-, and taxa-level diversity between AA and EA children’s gut and oral microbial diversity. On the one hand, in EA children, there was no difference in gut microbial diversity between obese and non-obese. At the same time, on the other side, there was a link between the abundance of gut Klebsiella and Magasphaera and obesity in AA children. In obese EA children, however, an excess of oral Aggregatibacter and Eikenella was discovered. These findings pointed out an ethnicity-specific link between gut and mouth microbial profiles. Microbiota in obesity was influenced by socioeconomic factors, which differed by ethnicity, implying that specialized measures to combat obesity are required for both populations.